Unraveling the molecular basis of dendrite morphogenesis is therefore an important goal. Growing evidence indicates that dendrite morphogenesis is a tightly regulated process and that perturbations of the genetic programs that orchestrate it can result in defects that manifest both at the circuit and behavioral levels ( Puram and Bonni, 2013 Dong et al., 2015). Morphogenesis of neuronal dendritic arbors influences neuronal connectivity and functional specialization and is thus a critical step in nervous system development. Together, our findings support a role for Basigin in complex dendrite morphogenesis and interactions between dendrites and the adjacent epidermis. Furthermore, knock down of Basigin in the epidermis led to defects in dendrite elaboration of class IV neurons, suggesting a non-autonomous role. Structure-function analyses showed that a juxtamembrane KRR motif is critical for this function. Classes of sensory neurons with simpler branching patterns were unaffected by loss of Basigin. Loss of Basigin in da neurons led to defects in morphogenesis of the complex dendrites of class IV da neurons. We found that reporters for Basigin, a cell surface molecule of the immunoglobulin (Ig) superfamily previously implicated in cell-cell and cell-substrate interactions, are expressed in da sensory neurons and epidermis. To identify potential mediators of neuron-substrate interactions important for dendrite morphogenesis, we undertook an expression pattern-based screen in Drosophila larvae, which revealed many proteins with expression in dendritic arborization (da) sensory neurons and in neurons and their epidermal substrate. However, the molecular basis of this important developmental process remains poorly understood. 3Department of Physiology and Cellular Biophysics, Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, United StatesĬoordination of dendrite growth with changes in the surrounding substrate occurs widely in the nervous system and is vital for establishing and maintaining neural circuits.2Department of Neuroscience, Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, United States.1Department of Neuroscience, Columbia University Medical Center, New York, NY, United States.Shrestha 1*†, Anita Burgos 2 and Wesley B.
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